Serodiagnosis of leprosy in patients' contacts by enzyme-linked immunosorbent assay
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چکیده
منابع مشابه
Evaluation of Enzyme Linked Immunosorbent Assay, Utilizing Native Antigen B for Serodiagnosis of Human Hydatidosis
Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...
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The sensitivity specificity of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of strongyloidiasis has been investigated. 45 men with long-standing strongyloidiasis were compared with the same number of age- and sex-matched control subjects. The ELISA detected antibody in 84% of patients with parasitologically proven strongyloidiasis. When the technique was compared with an i...
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An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملImmunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)
Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
متن کاملenzyme-linked immunosorbent assay
An enzyme-linked immunosorbent assay was used to titrate antisera raised against live cultures of eight type species (biotypes) of Bacteroides with the EDTA-released outer-membrane complex from 29 characterized strains of Bacteroides species. With only minor exceptions, the strains investigated reacted to titre with the antisera raised against the homologous type species and not against the het...
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ژورنال
عنوان ژورنال: Leprosy Review
سال: 1990
ISSN: 0305-7518
DOI: 10.5935/0305-7518.19900013